a 2025

THE IMPACT OF CYTOKININS AND CYTOKININ METABOLISM IN IN VITRO SHOOT REGENERATION

ŠMERINGAI, Ján and Markéta PERNISOVÁ

Basic information

Original name

THE IMPACT OF CYTOKININS AND CYTOKININ METABOLISM IN IN VITRO SHOOT REGENERATION

Authors

ŠMERINGAI, Ján and Markéta PERNISOVÁ

Edition

OP JAK tangenc TOWARDS NEXT GENERATION CROPS:FROM DISCOVERY TO APPLICATIONS, Telč, 2025

Other information

Language

English

Type of outcome

Konferenční abstrakta

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

Marked to be transferred to RIV

No

Organization

Středoevropský technologický institut – Repository – Repository

Keywords in English

Cytokinins; Auxin–cytokinin balance; In vitro shoot regeneration; AHK4 cytokinin receptor; WUSCHEL; transcription factor

Links

EH22_008/0004581, research and development project.
Changed: 17/3/2026 00:51, RNDr. Daniel Jakubík

Abstract

In the original language

The phytohormones cytokinins and auxin have been known as theprincipal regulators of plant development for a long time. In in vitroregeneration assays, the presence of high auxin-to-cytokininconcentration ratio in media induces root regeneration from variousplant tissues. On the other hand, if the auxin-to-cytokinin ratio is low,shoots are formed. The process of in vitro shoot regeneration is accompanied by strongactivation of cytokinin signalling via AHK4 receptor and induction ofshoot-specific homeodomain regulator WUSCHEL specifically in theregenerating organs. Exogenous but also endogenous cytokininsinfluence both the initiation of newly formed organs as well as thepace of organ developmental sequence. The role of cytokinins,importance of cytokinin biosynthesis and metabolism in the control ofin vitro shoot regeneration will be discussed.

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